Effect of PCR extension temperature on high-throughput sequencing
نویسندگان
چکیده
منابع مشابه
Effect of the enzyme and PCR conditions on the quality of high-throughput DNA sequencing results
Library preparation protocols for high-throughput DNA sequencing (HTS) include amplification steps in which errors can build up. In order to have confidence in the sequencing data, it is important to understand the effects of different Taq polymerases and PCR amplification protocols on the DNA molecules sequenced. We compared thirteen enzymes in three different marker systems: simple, single co...
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ژورنال
عنوان ژورنال: Molecular and Biochemical Parasitology
سال: 2011
ISSN: 0166-6851
DOI: 10.1016/j.molbiopara.2010.11.013